4 replies to this topic

[yobou]

Dar All
some antibodies (containing sodium  azide as a preservative) that should be stored at 4-8 oC were stored by mistake at -20oC for 24 hrs. Should this spoil them?
thanks

[klinmed]

yobou, on Mar 14 2009, 05:44 AM, said:

Dar All
some antibodies (containing sodium  azide as a preservative) that should be stored at 4-8 oC were stored by mistake at -20oC for 24 hrs. Should this spoil them?
thanks

Absolutely no problem.
For long-term storage antibodies are often stored at -20 oC.  One thing they do not like is MULTIPLE freeze/thaw cycles.
Just thaw your reagents, mix them gently, and store them at 4 oC, or in aliquots at -20 oC.
Hope this helps

[yobou]

I am worried because the manufacturer stated that (avoid freezing)

[klinmed]

yobou, on Mar 14 2009, 08:13 AM, said:

I am worried because the manufacturer stated that (avoid freezing)

The only potential problem could occur if your antibodies are IgM monoclonals. These are not commonly used because they have a tendency to aggregate especially after freezing.  If you are using polyclonals or IgG monoclonals I am pretty sure they will be fine. If you are REALLY worried you can always contact the manufacturer. Why not just try them. Am sure they will be fine

[mdfenko]

it could also be a problem if the antibody is conjugated to an enzyme (a secondary antibody). the enzyme may be sensitive to freezing.

the only way you will know for sure is to use the antibody and compare the result with the result from before freezing.