I am looking for a method to extract a protein from a coomassie/ silver stained gel. I am then going to digest it with tryp so I need it as concentrated as possible
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#2
anonymous
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Posted 14 July 2001 - 09:00 PM
Try Elutrap, it is a realy convenient method for electroelution of proteins from SDS-PAGE. But it works better if you don't make a strong staining procedure (try amido black staining).
You can find the description of the method in :[url="http://www.s-und-s.de/Pages-NEU-eng/UB3/Life%2520Science/Lab%2520Manual/labmanual.html"][url]http://www.s-und-s.de/Pages-NEU-eng/UB3/Li.../labmanual.html[/url][/url]
Good luck !
#3
leelaram
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Posted 21 July 2004 - 01:09 AM
hai,
instead of amido black staining. u can use CuCl2.H2) (0.3M) for negative staining, cut the unstained bands and destain with 0.25M tris and 0.25M EDTA
instead of amido black staining. u can use CuCl2.H2) (0.3M) for negative staining, cut the unstained bands and destain with 0.25M tris and 0.25M EDTA
M. Naga Leelaram,
PhD student,
Indian Institute of Science,
Bangalore
India
PhD student,
Indian Institute of Science,
Bangalore
India
