Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Dulbecco's PBS (Ca++, Mg++ free)


  • Please log in to reply
4 replies to this topic

#1 jehane

jehane

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 13 March 2009 - 02:51 PM

Dear all
could you please tell me how to prepare Dulbecco's PBS (Ca++, Mg++ free) ?? i need a recipe for preparing 1X solution in 1 Litre. Best regards.

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 14 March 2009 - 07:47 AM

it's a good question. I usually make my own PBS for normal lab usage, but for cell culture we use commercial mg- and ca-free PBS. I always wonder how they make it.

I buy 1xPBS from PAA. it's cheaper than SIGMA I guess.

#3 klinmed

klinmed

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 209 posts
1
Neutral

Posted 14 March 2009 - 08:34 AM

Dulbecco's Phosphate Buffered Saline (PBSA) - Dissolve 0.2 g of potassium chloride, 0.2 g of monobasic potassium phosphate (KH2PO4), 8 g of sodium chloride, and 1.74 g of dibasic sodium phosphate (Na2HPO4.7H20) in 0.9 liter of distilled (or Milli Q) water. Adjust the pH to 7.2 with concentrated sodium hydroxide if needed. Make up to 1 liter with water. Sterilize by autoclaving.

Be carefully to note the water of crystallization of the dibasic sodium phosphate you use. If the anhydrous salt you will need only 1.14 g.

There are alternative recipes. These usually differ slightly n the concentration of phosphate.

Edited by klinmed, 14 March 2009 - 09:06 AM.


#4 molgen

molgen

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 167 posts
0
Neutral

Posted 15 March 2009 - 02:19 AM

I like BIOLOGICAL INDUSTRIES because they give the formulations for all their products.
This link has on the bottom the formulation for PBS x1 or x10 if you need it.

#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,725 posts
399
Excellent

Posted 15 March 2009 - 03:31 PM

Note to all peoples that this is a PROTOCOLS site:

There is a page for protocols, linked right at the top of every page you visit. A quick search will find you what you need, especially for common solutions like PBS, Tris, etc.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.