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Phosphoproteins staining


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#1 BadKarma

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Posted 13 March 2009 - 10:44 AM

Dear all,

Does anyone have experience using ProQDiamond to stain phosphoproteins? I did my first experience with it and i'm not very happy with results... Look for some help to troubleshoot
"Ever tried. Ever failed. No matter. Try again. Fail again. Fail better." Samuel Becket

#2 lotus

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Posted 16 March 2009 - 01:38 PM

No I haven't used it. How about trying a generic phospho serine or phospghotyrosine antibody and doing a western blot.

#3 BadKarma

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Posted 19 March 2009 - 01:37 AM

Hi,

Antibodies are not the solution.
Were tested before using ant-phosphoSer, anti-phosphoThr and anti-phosphoTyr and recognize both unphosphorylated and phosphorylated forms.
We try ProQ Diamond expecting that solve our problems but no luck yet.

Thanks for reply.
"Ever tried. Ever failed. No matter. Try again. Fail again. Fail better." Samuel Becket




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