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IgG control for immunopercipitation


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#1 Brian Wynter

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Posted 13 March 2009 - 08:58 AM

Does anyone know why when performing immunopercipitation experiments IgG is used as a loading control as opposed to traditional controls (i.e. actin, GAPDH,tubulin)

Thanks

#2 Paraboxa

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Posted 13 March 2009 - 09:13 AM

You want a control that has similar characteristics to your target protein. IgG doesn't polymerize, whereas actin does. IgG is a glob whilst tubilin is long and stringy. Lastly, other Igs would polymerize (IgM, IgA).

Does that help.




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