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[Brian Wynter]

Does anyone know why when performing immunopercipitation experiments IgG is used as a loading control as opposed to traditional controls (i.e. actin, GAPDH,tubulin)

Thanks

[Paraboxa]

You want a control that has similar characteristics to your target protein. IgG doesn't polymerize, whereas actin does. IgG is a glob whilst tubilin is long and stringy. Lastly, other Igs would polymerize (IgM, IgA).  

Does that help.