Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

VEGF for western blot


  • Please log in to reply
2 replies to this topic

#1 ASC

ASC

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 13 March 2009 - 08:09 AM

Hello,

I was wondering whether anyone has done western blots to look for VEGF in human cells? If you have, then did you collect the media from the cells, precipitate the proteins using 80% acetone and then ran a sample of that or did you collect the lysates? I have tried it both ways, although I am not surprised that I didn't see VEGF in whole cell lysates as it is being secreted from the cells. Any help is appreciated.

Thanks.

#2 yobou

yobou

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 89 posts
1
Neutral

Posted 13 March 2009 - 10:54 PM

A colleague in my lab used ELISA to sucessfully detect it

#3 alwayslearning

alwayslearning

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 26 February 2010 - 10:37 AM

A colleague in my lab used ELISA to sucessfully detect it


I'm trying to detect VEGF in media from HUVECs using Quantikine ELISA from R&D systems but have had no success. Very frustrating, and Western blots of HUVEC lysate using Santa Cruz Ab also doesn't detect anything.

Has anyone had any luck with VEGF in HUVECs? :P




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.