Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

growth of colonies after ligation


  • Please log in to reply
2 replies to this topic

#1 sagar

sagar

    genes

  • Active Members
  • PipPipPipPipPip
  • 38 posts
0
Neutral

Posted 12 March 2009 - 09:32 AM

hello guys
i have a very curious question.i did several set of ligation using the same set of plasmid and the insert and did transformation.i tried to screen colonies containing the ligated product in amp plate.after12 hrs of incubation i didnot get any colonies but i just kept these plates on my desk and after 1 day i saw colonies in 3 plates but no colonies in the rest of the 2 plates.
how come that i got colonies in 3 plates ? is the mode of action of amp bacteriostatic? i dont understand how can colonies develop like this from no where.do the cells grow after the amp degrades in the plate?

#2 shimshady

shimshady

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
0
Neutral

Posted 12 March 2009 - 09:40 AM

Bacteria from your lunch on your desk got onto to the plates!

#3 sagar

sagar

    genes

  • Active Members
  • PipPipPipPipPip
  • 38 posts
0
Neutral

Posted 12 March 2009 - 10:21 AM

Bacteria from your lunch on your desk got onto to the plates!

thanks




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.