Trypsinized cells after fixation
Posted 10 March 2009 - 04:08 PM
Do you know if I can trypsinized the cells after fixation in order to obtain RNAm for RTPCR?
I really appreciate your help.
Thanks in advance.
Posted 09 August 2009 - 09:00 PM
Why do you fixate cells before extracting (total) RNA?
I've always isolated total RNA from cells with a MN nucleospin column and then used a one- or two step RT kit with oligo dT primers for q-RT PCR.
Posted 10 August 2009 - 05:13 PM
Posted 21 August 2009 - 03:47 AM