Accidentally digested DNA overnight - still OK?
Posted 10 March 2009 - 03:31 PM
Posted 10 March 2009 - 05:05 PM
Why not try a QPCR and see? You'll never know unless you give it a go.
Posted 11 March 2009 - 03:42 AM
commerically available enzymes, particular EcoRI,HindIII etc, are quite robust and show very little star activity under routine digestion conditions.
Posted 11 March 2009 - 07:09 AM
I routinely digest things overnight. As long as it's not an enzyme that generally shows star activity you should be fine.
Posted 12 March 2009 - 03:00 AM
Its okay........I also go for overnight digestions.