Shelf life of ampicillin in LB media?
Posted 10 March 2009 - 10:18 AM
Posted 10 March 2009 - 10:23 AM
i am pretty sure ampicillin plates do not degrade overnight...for that reason we can effeciently carry out transformation.well things do not turn up good all the time. I can safely comment that it does not get degraded overnight.
Edited by tuhin_genes, 10 March 2009 - 10:25 AM.
Posted 10 March 2009 - 10:44 AM
Posted 10 March 2009 - 10:56 AM
Posted 11 March 2009 - 01:44 AM
logic is good.may be for that reason we get sometimes false colonies.
Posted 16 March 2009 - 04:19 AM
I kept my Amp plates for almost 1 month at 4°C and they stayed selective. Also incubation for more then 2 days at 30°C did not influence selectivity so the antibiotic should be ok. Some papers say that you should not keep these plates for more than one week but from my experience they still work if you store them at 4°C. Good luck!
Posted 16 March 2009 - 11:54 AM
I observed these little sattelite colonies klinmed had mentioned before, but it took them about a week to grow. One night at 37C is safe.
If you actually have troubles with selectivity, than perhapse you should increase Amp concentration.