protein concentration method
Posted 10 March 2009 - 07:45 AM
I don't want to lose any of my protein. It could be denatured.
What would you recommend?
I'm thinking of TCA ppt and ultrafiltration. If you have better idea, please share.
Thank you in advance for your tips of advice.
Posted 10 March 2009 - 08:08 AM
I use TCA when I want to do SDS-PAGE (I resuspend the pellet in laemmli buffer)
Posted 10 March 2009 - 05:59 PM
Posted 12 March 2009 - 02:49 AM
I don't like to denature my protein and hate concentrators as the protein sticks to the membrane and I loose out a lot. I generally use anion exchange chromatography and concentrate it by using a running gradient. However, in case that doesn't work, I would transfer the protein in a dialysis bag with a cut-off and then hang it in the cold room for the buffer to drain off.
It sounds good to me but have never used it as FPLC works in most of my proteins.
If you use it and it works for you, I would appreciate if you could send me a message.
Posted 12 March 2009 - 02:48 PM