I am using TaqMan MGB probes and I get negative fluorescence signals during monitoring of my reaction between -10,000 to -200,000. Please help
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madrius1
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Posted 10 March 2009 - 06:56 AM
Is any contamination with a DNAse possible?
Also, some reagents may precipitate as the run goes.
Also, some reagents may precipitate as the run goes.
#3
kate
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Posted 11 March 2009 - 08:22 PM
Thank you for your feed back.
How I can check the contamination of DNAase?
Is there any possibility of poor probe design? What parameters should I check?
Thank you.
How I can check the contamination of DNAase?
Is there any possibility of poor probe design? What parameters should I check?
Thank you.
madrius1, on Mar 10 2009, 06:56 AM, said:
Is any contamination with a DNAse possible?
Also, some reagents may precipitate as the run goes.
Also, some reagents may precipitate as the run goes.
