Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Negative flouroscence in real time PCR


  • Please log in to reply
2 replies to this topic

#1 kate

kate

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 10 March 2009 - 01:12 AM

I am using TaqMan MGB probes and I get negative fluorescence signals during monitoring of my reaction between -10,000 to -200,000. Please help

#2 madrius1

madrius1

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 114 posts
0
Neutral

Posted 10 March 2009 - 06:56 AM

Is any contamination with a DNAse possible?

Also, some reagents may precipitate as the run goes.

#3 kate

kate

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 11 March 2009 - 08:22 PM

Thank you for your feed back.

How I can check the contamination of DNAase?

Is there any possibility of poor probe design? What parameters should I check?

Thank you.



Is any contamination with a DNAse possible?

Also, some reagents may precipitate as the run goes.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.