Gencarrier-1 vs. CaPO4 transfections
Posted 09 March 2009 - 11:55 AM
I purchased Gancarrier-1 and used it to transfect HeLa cells with pGL-3 vectors for a few time. No luminescence has ever been detected at all. Then I switched to CaPO4 method. For the first time I got some luminiscence, although the conditions might need to be further optimized to improve the efficience. Now my question is: Is CaPO4 method only suitable for certain cell lines and certain studies? My boss doubt that Ca would trigger the signalling of some pathways in these cells.
On the other hand, I still don't know why my transfections with Gencarrier-1 always failed. Can somebody tell me whether there is some trick for your success of utilizing this reagent? Thank you so much!!!
Posted 11 March 2009 - 01:56 AM
Posted 11 March 2009 - 06:17 AM
But you might want to try some other reagents that have been shown to work well with your line.
Posted 12 March 2009 - 03:58 PM
Many of the protocols for these reagents are based on area of plate or dish rather than the amount of DNA being transfected, so check your conditions and see what you did.
Posted 15 March 2009 - 07:26 PM