Posted 12 September 2001 - 09:00 PM
You may have a hard time with the methylmercury method, since methylmercuric hydroxide is not longer commercially available and is very toxic. However, look back at a message from 14 Aug. 2000 in this forum under the same subject. One solution suggested there was to use 18 mM dGdC dinucleotide in the reaction. I have not tried it but it seems to me that should bind to G's and C's in your mRNA without blocking the binding site of your oligo-dT primer. The polymerase should be able to remove the dinucleotides during the polymerization process.