help! Truncated protein cannot expressed in mammalian cells?
Posted 09 March 2009 - 09:53 AM
Posted 09 March 2009 - 10:42 AM
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Thomas Henry Huxley
Posted 09 March 2009 - 10:59 AM
Dr Teeth, thanks for your reply. My target antibody only detect the c-terminal part of the protein, which means it won't recognize the truncated protein when I delet the c-terminal. For one of the constructs I retained the c-terminal, that antibody couldn't work either. The thing is wether I delet the N or C or even a middle part of the protein, I cannot detect anything. Even when I pull-down with protein G, it didn't show anything. BTW, I fused the proetin fragments with a GFP N-terminal tag as well, it seems the expression of GFP-fused proteins have no problem. I guess I will try the in vitro transcription, but in that way, I cannot do my following experiments.
Posted 21 April 2009 - 02:23 PM
Posted 22 April 2009 - 09:35 AM
Posted 24 April 2009 - 08:43 PM
Understanding Life One Protein at a Time...
Posted 16 November 2012 - 05:42 AM
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