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stabilization of purified protein solutions


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#1 paramyosin

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Posted 09 March 2009 - 03:19 AM

I have several problems with protein solutions hughly purified, such us aggregation. Any of you could give any tip about agents for stabilization of these kind of solutions?. I am trying: NaCl, DTT, detergent, sugars, pH modification but i am sure there should be quite more.
It would be also very usefull some webpage regarding stabilization of protein solutions, i am looking out for some of them but i have´t found a real good one.
Any suggestions?
Thank you in advance
"Research without indebtedness is suspect, and somebody must always, somehow, be thanked." Umberto Eco

#2 mdfenko

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Posted 09 March 2009 - 12:39 PM

we used peg-8000 (0.5-5%) to prevent aggregation of some proteases with which we worked.

be careful, higher amounts (~20%) will precipitate proteins and it will co-concentrate with the protein when concentrating by ultrafiltration with a relatively low mw cutoff (10 kDa).
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#3 paramyosin

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Posted 10 March 2009 - 01:50 AM

Thank you mdfenko, i will try PEG. For some proteins i have used nearly everything to avoid aggregation in certain conditions
Thank you very much
"Research without indebtedness is suspect, and somebody must always, somehow, be thanked." Umberto Eco




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