Western blot has alot of spots
Posted 08 March 2009 - 10:09 PM
Have any of you guys had the same problem?
Posted 09 March 2009 - 12:41 AM
Posted 09 March 2009 - 02:13 AM
i used the same SDS for my beta actin control but there is no problem there. I will prepare new sds anyway. thanka
I remember that someone has such problem. He solved the problem by preparing new SDS solution.
Posted 09 March 2009 - 04:04 AM
Posted 09 March 2009 - 04:09 AM
if you strip this membrane and blot with your antibody of interest, you have a starry sky.
The same with a membrane freshly transferred and blotted with your antibody of interest.
if yes, don't lose time with preparing new SDS, it is not responsable for your problems.
what do you use as blocking reagent? milk?
do you blot against phospho-protein? biotinylated protein?
Edited by little mouse, 09 March 2009 - 04:11 AM.
Posted 13 March 2009 - 09:08 PM