6 replies to this topic

[thinsu]

Hi All!
Please help me.
I use RNAsed free water and 70% ethanol and UV for 1 hour for decontamination for PCR.Is it enough?
And which is better to use distilled water or RNAsed free water and UV for 1 hour & 70% ethanol and UV for 1 hour between viral load testings?
Thanks a lot
Thin Su

[Felipillo]

May be this old discussion could help you.

[bob1]

For removal of DNA, a bleach solution (1%) will be the most effective.  For removal of viruses, wipe the surfaces with Trigene or Virkon., then 70% ethanol.

[NemomeN]

If cross contamination is a concern, use filter tips as well.

[thinsu]

bob1, on Mar 8 2009, 04:41 PM, said:

For removal of DNA, a bleach solution (1%) will be the most effective.  For removal of viruses, wipe the surfaces with Trigene or Virkon., then 70% ethanol.

Thank you.I will try it.

[thinsu]

Déjà Vu, on Mar 8 2009, 07:40 AM, said:

May be this old discussion could help you.

Thanks a lot.

[thinsu]

NemomeN, on Mar 8 2009, 07:27 PM, said:

If cross contamination is a concern, use filter tips as well.

Thank.I use only filter tips.