Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

decontamination


  • Please log in to reply
6 replies to this topic

#1 thinsu

thinsu

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 08 March 2009 - 06:22 AM

Hi All!
Please help me.
I use RNAsed free water and 70% ethanol and UV for 1 hour for decontamination for PCR.Is it enough?
And which is better to use distilled water or RNAsed free water and UV for 1 hour & 70% ethanol and UV for 1 hour between viral load testings?
Thanks a lot
Thin Su

#2 Felipillo

Felipillo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
4
Neutral

Posted 08 March 2009 - 06:40 AM

May be this old discussion could help you.
Chance favors the prepared mind
Louis Pasteur.

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,830 posts
414
Excellent

Posted 08 March 2009 - 03:41 PM

For removal of DNA, a bleach solution (1%) will be the most effective. For removal of viruses, wipe the surfaces with Trigene or Virkon., then 70% ethanol.

#4 NemomeN

NemomeN

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 34 posts
0
Neutral

Posted 08 March 2009 - 06:27 PM

If cross contamination is a concern, use filter tips as well.

#5 thinsu

thinsu

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 11 March 2009 - 12:50 AM

For removal of DNA, a bleach solution (1%) will be the most effective. For removal of viruses, wipe the surfaces with Trigene or Virkon., then 70% ethanol.

Thank you.I will try it.

#6 thinsu

thinsu

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 11 March 2009 - 12:51 AM

May be this old discussion could help you.

Thanks a lot.

#7 thinsu

thinsu

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 11 March 2009 - 12:52 AM

If cross contamination is a concern, use filter tips as well.

Thank.I use only filter tips.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.