Troubleshoot ARMS PCR
Posted 08 March 2009 - 06:01 AM
I'm using ARMS PCR method to detect common mutations in beta thalassaemia samples. PCR product for certain samples seems to be fine but having difficulty in amplifying some samples for the same mutation analysis. Can anyone advise me pls. thank you.
Posted 09 March 2009 - 03:38 AM
Do you use similar DNA concentration? Is your DNA a good quality? You can do some check on a different gene, like actin for example.
If everything seems fine, and you miss the products there may be some sort of deletion in the area of your primers, especially when you look at thalassaemia pacients.
I never trust anything that can't be doubted.
Posted 02 September 2010 - 12:24 AM