2 replies to this topic

[SF_HK]

Hi,

What kind of vector is pCS2-flag vector. Can it be used to overexpression a gene in mammalian cells/ stable transfection?

[molgen]

SF_HK, on Mar 8 2009, 07:19 AM, said:

Hi,

What kind of vector is pCS2-flag vector. Can it be used to overexpression a gene in mammalian cells/ stable transfection?

To answer your question go to Addgene. You can find a lot of info about vectors in that database.

As it says there, the vector pCS2-flag is a Bacterial expression vector.

[NemomeN]

I do not know if the pCS-flag vector you are referring to is the same as the pCS2+ series, but if so, then yes, they are suitable fore expression in mammalian cells.  It contains the CMV promoter.