Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

shRNA transgenic mice

  • Please log in to reply
1 reply to this topic

#1 mateo



  • Members
  • Pip
  • 2 posts

Posted 07 March 2009 - 02:15 PM

Hello. Does anyone have any information or advice on making mice with tissue specific overexpression of shRNA? Ideally my gene of interest would be floxed and then crossing on the tissue specific Cre mouse would be my first option, however that project will take a year or two. Making a transgenic shRNA mouse would be much faster but I've only seem a couple of papers that have used this strategy. Additionally, lentiviral delivery is not an option because they don't transfect the lung epithelium-which is where I want my knockdown.
Any insight would be greatly appreciated.

#2 Jon Moulton

Jon Moulton


  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 142 posts

Posted 09 March 2009 - 01:30 PM

This just out:

Generation of shRNA Transgenic Mice.

Hitz C, Steuber-Buchberger P, Delic S, Wurst W, Kühn R.

Methods Mol Biol. 2009;530:1-29.

Jon D. Moulton
Gene Tools, LLC

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.