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negative control for PCR


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#1 elen

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Posted 07 March 2009 - 06:54 AM

hi

if I use different set of primers and one DNA template, should I have one negative control for every primer pair?
if so, why? or is it enough to just have one neg. control with one primer pair?

thanks

#2 tea-test

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Posted 07 March 2009 - 07:11 AM

you should have one for every primer pair because also your primers could be contaminated.
tea-test: The artist formerly known as Ned Land




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