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Rcovery of proteins from gels


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#1 Advir

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Posted 06 March 2009 - 12:46 PM

I wonder if anyone knows of a way to recover proteins from an electrophorsis gel?????

#2 T C

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Posted 06 March 2009 - 08:59 PM

Hey

Cut the band out, put it in a dialysis tube with a cut off less than the size of yr protein and fill with buffer, put it in a tank and apply voltage. The protein will move out of the gel but stay inside the dilysis tubing, take it out, concentrate and use.

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TC

#3 mdfenko

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Posted 09 March 2009 - 12:48 PM

or you could mash the gel and wash out the protein with buffer (and a centrifuge, and you could use a centrifugal filter instead of pelleting and decanting) then concentrate.
talent does what it can
genius does what it must
i do what i get paid to do




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