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IEF buffer (cathod and anode) for range 4-6


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#1 rick112

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Posted 06 March 2009 - 08:51 AM

hi

i trying to develop a IEF method using ampholytes 4-6 can anyone suggest suitable Buffers/electrolytes (for anode and cathode) ???

thanks a lot

#2 neuron

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Posted 06 March 2009 - 09:13 AM

Hi,

I dint get your question, Are you talking about rehydration buffer? Generally the sample is dissolved in rehydration buffer which contains urea, thiourea and CHAPS.

#3 rick112

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Posted 06 March 2009 - 07:35 PM

hi amj2 ,

what i would like to know is that for given range of ampholytes (4-6) what anode and cathod buffers should i use for running the IEF??

(note:- i am not doing IPG strip...my experiment is simple "Isoelectric focusing" using carrier ampholytes)

thanks a lot

#4 mdfenko

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Posted 09 March 2009 - 12:26 PM

according to the information available from ge healthcare, acetic acid for the anode and naoh for the cathode (0.5M each).

see this manual.

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