Cloning multiple miR30 based shRNAs into a Lenti
Posted 06 March 2009 - 05:55 AM
I want to clone in a reporter (mCerulean/tdTomato) and three different shRNAs in a mir30 format.
1. can i use just the one promoter, or do i have to use two?
2. do i have to separate the miR30s by a stretch of sequence?
3. is this fundamentally flawed???
Any help would be great!
Posted 17 March 2009 - 09:54 PM
Why do you want to clone multiple copy of the same miRNA? want to obtain high expression?
Posted 03 April 2009 - 08:11 PM