3 replies to this topic

[SF_HK]

Hi,

In our lab we usually stain the gel with EtBr solution after electrphoresis. However, teh person who make sthis solution is on a conference ad I need to prepare a fresh solution.  I vaguley remember him telling me to add 40ul EtBr solution in 400 ml TAe buffer.  HOw  do other people make their solution?

[little mouse]

I don't put it in electrophoresis buffer, but in the gel. I do a 10,000 dilution also. (40µL  in 400 mL)

[HomeBrew]

It would depend, of course, on the concentration of your stock EtBr solution.  Those recipes sound like they're assuming a stock concentration of 10 mg/ml.  In any event, it's not *that* critical -- just add EtBr to the TAE until the solution is approximately as dark as it usually is when the person who's away makes it...

[scolix]

We also add to EtBr to gel. I use 2ul of 10mg/ml EtBr to a 50ml gel.