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EtBr solution


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3 replies to this topic

#1 SF_HK

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Posted 05 March 2009 - 11:41 PM

Hi,

In our lab we usually stain the gel with EtBr solution after electrphoresis. However, teh person who make sthis solution is on a conference ad I need to prepare a fresh solution. I vaguley remember him telling me to add 40ul EtBr solution in 400 ml TAe buffer. HOw do other people make their solution?

#2 little mouse

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Posted 06 March 2009 - 02:33 AM

I don't put it in electrophoresis buffer, but in the gel. I do a 10,000 dilution also. (40ÁL in 400 mL)

#3 HomeBrew

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Posted 06 March 2009 - 05:36 AM

It would depend, of course, on the concentration of your stock EtBr solution. Those recipes sound like they're assuming a stock concentration of 10 mg/ml. In any event, it's not *that* critical -- just add EtBr to the TAE until the solution is approximately as dark as it usually is when the person who's away makes it...

#4 scolix

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Posted 07 March 2009 - 04:07 AM

We also add to EtBr to gel. I use 2ul of 10mg/ml EtBr to a 50ml gel.




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