Posted 05 March 2009 - 02:35 PM
I'm running western blots for some proteins in pancreatic cyst fluid.
I'm not very familiar with cyst fluid at this point. Is there any way to
test for blood contamination in my samples? I'm concerned about blood
proteins affecting my results.
Posted 05 March 2009 - 03:56 PM
Posted 15 September 2018 - 11:51 PM
Hemoglobin urine micromethod assay
- TMB solution: dissolve 2 mg of 3’,3’-5’-5’-tetramethylbenzidine in 20 ml of 0.05 M acetic acid (2.862 ml of glacial acetic acid to 1 liter dH2O. For 20 ml, dilute 60 µl of glacial acetic acid with 19.94 ml dH2O)
- 30% Peridrole
- Working solution: mix 300 µl of TMB solution with 2 µl of peridrole. Stable one hour at room temperature.
Dispense in well MTP, 200 µl of urine and then add 75 µl of working solution and incubate at room temperature for 10 min. A blue color intensity is correlate with peroxidase activity present in urine.
This method can be used in all biological fluids.