Phospatase inhibitors needed for LDS Buffer?
Posted 05 March 2009 - 02:13 PM
I am trying to look at the phosphorylation of the gene c-Met. I do not have a lot of experience with Westerns, however another lab is helping me out and they tell me to lyse cells in laemmli sample buffer and it stops all reactions including phophatases. However I have read in most cases people use RIPA buffers supplemented with protease and phophatase inhbitors.
Is it true that LDS sample buffer will stop all reactions including phosphatases which might cause the dephosphorylation of the gene I am trying to look at, C-met. Thanks
Posted 06 March 2009 - 08:51 AM
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Posted 13 March 2009 - 09:11 PM
Posted 25 September 2009 - 05:12 AM
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