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Explants used in direct somatic embryogenesis


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#1 QQ_lin

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Posted 05 March 2009 - 09:32 AM

Can we choose root as explant when performing direct somatic embryogenesis for plant tissue culture? What are the other forms of explants that we can use for direct somatic embryogenesis? Thanks... :)

#2 bob1

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Posted 08 March 2009 - 03:53 PM

Looks like homework to me... have you tried this link

#3 QQ_lin

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Posted 08 March 2009 - 05:11 PM

Looks like homework to me... have you tried this link



I have tried to search from there so many times....But still cannot get the Confirmed answers...Anyways thanks for your reply... :P

Can anyone help me... :P

#4 bob1

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Posted 09 March 2009 - 03:43 PM

Looks like you need meristem for it work, so you could use parts of the roots, but then I haven't done it or studied it

#5 QQ_lin

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Posted 10 March 2009 - 07:39 AM

Looks like you need meristem for it work, so you could use parts of the roots, but then I haven't done it or studied it



Most of the journals that I have read are using cotyledons from seeds. If I directly used roots to perform the tissue culture, the techniques used seems to be organogenesis instead of direct somatic embryogenesis is it? I am confuse about it... :huh:

#6 robbiebc

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Posted 24 March 2009 - 02:59 AM

The roots, stem, leaf, meristem and other parts of plants can grow via direct somatic embryogenesis if the media and plant growth regulators used are right. However, you may need to try out which explants will give you the best result as different plants will give the different result.

Good luck

#7 QQ_lin

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Posted 24 March 2009 - 09:36 AM

The roots, stem, leaf, meristem and other parts of plants can grow via direct somatic embryogenesis if the media and plant growth regulators used are right. However, you may need to try out which explants will give you the best result as different plants will give the different result.

Good luck



I am now using the fully matured cotyledon parts from the zygotic embryo of the seed to perform the tissue culture. Thanks for your information. I appreciate it. :(




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