1 reply to this topic

[napstoo]

Hi all,

Currently extracting DNA from v. old blood (10 years old) so quality not great as you might expect. Am planning to run the DNA on a ABI3730 sequencer.

I am using the Ambion glycogen carrier to improve yields. 260/280 ratios are acceptable but 260/230 ratios are very poor often <1.0. Was wondering if the glycogen carrier has anything to do with this and if so is there a way to remove it afterwards?

Regards,

Ben

[mdfenko]

just going through some of the literature (found in a google search), it appears that glycogen requires iodine to absorb in the uv-vis range.

so it is probably not responsible for your low 260/230 ratio.

Edited by mdfenko, 04 March 2009 - 08:24 AM.