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how to deal with PVDF filter after WB


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5 replies to this topic

#1 lufang

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Posted 04 March 2009 - 12:33 AM

after western blot, if i can store the PVDF and use again, how to deal with the filter before i store it? thanks.

#2 Nrelo

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Posted 04 March 2009 - 03:52 AM

after western blot, if i can store the PVDF and use again, how to deal with the filter before i store it? thanks.


usually i will immediately rinse the membrane with wash buffer after blotting and then block the membrane with 5% BSA. I keep the membrane at 4C and it is fine for several days, although the colour of pre-stained marker gradually faded

#3 Lusen

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Posted 04 March 2009 - 07:19 AM

after western blot, if i can store the PVDF and use again, how to deal with the filter before i store it? thanks.


If you want to store it, I have usually dipped it in pure methanol, dried it on a piece of filterpaper and stored it in a plastic bag in the fridge. Dip in methnol to rewet it before you proceed with your blotting procedure. I have used this to store membranes for a couple of days, but it may last longer.

#4 MaggieRoara

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Posted 08 March 2009 - 10:04 PM

after western blot, if i can store the PVDF and use again, how to deal with the filter before i store it? thanks.


If you want to store it, I have usually dipped it in pure methanol, dried it on a piece of filterpaper and stored it in a plastic bag in the fridge. Dip in methnol to rewet it before you proceed with your blotting procedure. I have used this to store membranes for a couple of days, but it may last longer.


i do this too. It works fine for me.
For nitrocellulose membrane. just do the same but with water

#5 Lusen

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Posted 10 March 2009 - 10:36 AM

after western blot, if i can store the PVDF and use again, how to deal with the filter before i store it? thanks.


If you want to store it, I have usually dipped it in pure methanol, dried it on a piece of filterpaper and stored it in a plastic bag in the fridge. Dip in methnol to rewet it before you proceed with your blotting procedure. I have used this to store membranes for a couple of days, but it may last longer.


i do this too. It works fine for me.
For nitrocellulose membrane. just do the same but with water


Thanks, that is good to know. My old lab used PVDF, but my new one nitrocellulose and I never knew if it was possible to dry and use the nitrocellulose later

#6 yobou

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Posted 13 March 2009 - 09:04 PM

I just leave it as such after filming it at referegerator. Sometime I wash it with TBS buffer and store at referegerator.




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