Plasmid extraction sans commercial kits
Posted 03 March 2009 - 07:14 PM
Can anyone link me to sites with protocols for plasmid extraction without the use of kits?
Commercially, there are kits for the extraction of the genomic DNA and also the plasmid but what separates these two kits? i.e. how is it that one gives the genomic DNA and the other, plasmids?
Cell lysis > Protein precipitation > Transfer into isopropanol > Ethanol > Reconstitute
Thanks a lot!
Posted 03 March 2009 - 07:39 PM
Posted 04 March 2009 - 11:55 AM
Your lab must have the "Molecular Cloning" books.
Every lab has them. Or maybe "Current Protocols".
If you do not have them, get them and read them.
EVERY lab in the world has them.
They have great protocols for plasmid purification.
I don't recommend Cesium Chloride because it is time consuming.
I do recommend the Polyetylene Glycol method. Not as pure in general but much
Here's a link for these books at Amazon. It is a 3 volume set. Get them.