I did transformation with E coli then I did cell cultre.... miniprep...restriction enzyme digest then 1% agarose gel electrophoresis
as you have seen in the gel i repeated this experiment two time one of them duplicate for two insert with same plasmid but I got a strange result as I enclosed in the photo
could you help me find what is the problem? I try to do every thing ideally....
Visit My Website lane 2 and 3 the same plasmid with the same insert lane 4 and 5 the same plasmid with different insert
Visit My Website lane 2 is the same as lane &3 above , lane 3 is the same as lane 4&5 above
Sorry I can't upload the photos on this page"
Thank you in advance
Edited by saad, 03 March 2009 - 04:45 PM.