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artifacts in inmunofluorescence

Started by sela, Mar 03 2009 02:48 PM

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#1 sela

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Posted 03 March 2009 - 02:48 PM

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?

#2 bob1

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Posted 03 March 2009 - 03:08 PM

Paraformaldehyde (PFA) can cause artifacts through autoflourescence of undissolved PFA crystals.

#3 rkay447

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Posted 03 March 2009 - 03:31 PM

View Postsela, on Mar 3 2009, 03:48 PM, said:

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?
There are papers showing that certain fixations can influence the localization and staining of a specific protein.  Check out this paper: PMID: 1527176
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