Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

artifacts in inmunofluorescence

Started by sela, Mar 03 2009 02:48 PM

  • Please log in to reply
2 replies to this topic

#1 sela

sela

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 March 2009 - 02:48 PM

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 4,340 posts
222
Excellent

Posted 03 March 2009 - 03:08 PM

Paraformaldehyde (PFA) can cause artifacts through autoflourescence of undissolved PFA crystals.

#3 rkay447

rkay447

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 165 posts
19
Good

Posted 03 March 2009 - 03:31 PM

View Postsela, on Mar 3 2009, 03:48 PM, said:

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?
There are papers showing that certain fixations can influence the localization and staining of a specific protein.  Check out this paper: PMID: 1527176
Back to General Lab Techniques · Next Unread Topic →


  1. BioForum
  2. → Protocols and Techniques Forums
  3. → General Lab Techniques
  4. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.