Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

artifacts in inmunofluorescence


  • Please log in to reply
2 replies to this topic

#1 sela

sela

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 March 2009 - 02:48 PM

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,736 posts
400
Excellent

Posted 03 March 2009 - 03:08 PM

Paraformaldehyde (PFA) can cause artifacts through autoflourescence of undissolved PFA crystals.

#3 rkay447

rkay447

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 180 posts
20
Excellent

Posted 03 March 2009 - 03:31 PM

Hi all,
Does anybody know if methanol or PF and methanol introduces artifacts of some kind in inmunofluorescence?

There are papers showing that certain fixations can influence the localization and staining of a specific protein. Check out this paper: PMID: 1527176




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.