I want to use quick change kit to make site-directed mutagenesis. But becuae I am in a small protein crystallography lab, my boss told me that we can't afford the expensive kit. So I want to know where can I find the articles describing this method? Or can we use ordinary Taq polymerase instead to carry on my work? Any beneficial suggestions will be very appreciated.
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milanoj
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Posted 23 March 2002 - 06:20 AM
b.r.,
There are 7 references at the end of the protocol for quick change. Try Stratagene's web site for the protocol. It would be a pain to list them here. If you can avoid it use a thermostable polymerase that has 3'-5' exo activity. Try to avoid taq. Perhaps you could talk a company into giving you a free sample.
There are 7 references at the end of the protocol for quick change. Try Stratagene's web site for the protocol. It would be a pain to list them here. If you can avoid it use a thermostable polymerase that has 3'-5' exo activity. Try to avoid taq. Perhaps you could talk a company into giving you a free sample.
