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Progesterone in cell culture - are my levels correct?


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#1 AussieUSA

AussieUSA

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Posted 03 March 2009 - 08:31 AM

I am using progesterone in cell culture and want to use levels equivalent to human menstrual phases and human pregnancy.

Most data for human levels is presented in ng/ml such that:

luteal phase - 1-1.5 ng/ml
follicular phase - 2-28 ng/ml
1st trimester - 9-47 ng/ml
2nd trimester - 17-147 ng/ml
3rd trimester - 55-200 ng/ml

But in cell culture we use concentration (nM).

If the molecular weight of progesterone is 314.47 g/mol than after calculating concentrations I will assume the following:

luteal phase - 3-5 nM
follicular phase - 6-100 nM
1st trimester - 30-200 nM
2nd trimester - 150-450 nM
3rd trimester - 165-600 nM

Do these concentrations look correct? (i.e. I don't need verification that my calculations are correct, just that the concentrations match assumed human levels)

Thanks,

AussieUSA.




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