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How much R^2 that can be accept for standard curve of Bradford's assay?


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4 replies to this topic

#1 MT-TIKY

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Posted 03 March 2009 - 02:00 AM

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

#2 T C

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Posted 03 March 2009 - 02:23 AM

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?



#3 MT-TIKY

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Posted 03 March 2009 - 03:01 AM

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?




THANKS :(

#4 Nrelo

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Posted 03 March 2009 - 05:06 AM

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?


lowry has a higher chemical competibility than bradford

#5 bob1

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Posted 03 March 2009 - 03:27 PM

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?


It depends: Does the line fit the data (i.e. would a curve fit better?)? What are your residual plots like - random, non-random? Do you have any points on your standard curve outside of the given sensitivities of the Bradford assay?




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