I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?
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How much R^2 that can be accept for standard curve of Bradford's assay?
Started by MT-TIKY, Mar 03 2009 02:00 AM
4 replies to this topic
#2
T C
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Posted 03 March 2009 - 02:23 AM
Yup it is
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
MT-TIKY, on Mar 3 2009, 03:30 PM, said:
I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?
#3
MT-TIKY
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Posted 03 March 2009 - 03:01 AM
T C, on Mar 3 2009, 05:23 PM, said:
Yup it is
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
MT-TIKY, on Mar 3 2009, 03:30 PM, said:
I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?
THANKS
#4
Nrelo
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Posted 03 March 2009 - 05:06 AM
T C, on Mar 3 2009, 02:23 AM, said:
Yup it is
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
ButI like BCA or Lowry more thn Bradford for protein estimation.
TC
MT-TIKY, on Mar 3 2009, 03:30 PM, said:
I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?
lowry has a higher chemical competibility than bradford
#5
bob1
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Posted 03 March 2009 - 03:27 PM
MT-TIKY, on Mar 3 2009, 03:00 AM, said:
I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?
It depends: Does the line fit the data (i.e. would a curve fit better?)? What are your residual plots like - random, non-random? Do you have any points on your standard curve outside of the given sensitivities of the Bradford assay?
