1 reply to this topic

[jiro_killua]

I need to clone a gene and transfect into human cell line,

I need to tag my protein with Myc tag (N or C terminal both ok)

And express with a CMV promoter

Hopefully I want to insert the gene into the vector using the cutting site: 5' EcoRI, 3' SalI

Does anyone know of a vector that I can use?

Edited by jiro_killua, 02 March 2009 - 08:01 PM.

[scolix]

One could PCR amplify the gene with the myc tag.