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can we use one-step RT-PCR kits to amplify DNA?


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#1 Curtis

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Posted 02 March 2009 - 07:35 PM

hi,

I just want to know if it is possible to use one-step RT-PCR kits to amplify the DNA samples?

I have a one-step kit in the lab, but don't have any other normal PCR kits. just wondering if I could use it to amplify my DNA sample. as long as I don't have RNA in the sample then no cDNA will be made so it shouldn't be making any problem. I could even skip programming for cDNA making on the machine.....has anybody done this before?

#2 phage434

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Posted 02 March 2009 - 08:49 PM

This would probably work, but it seems a bit of a shame to waste the more expensive kit on a normal PCR.




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