1 reply to this topic

[Curtis]

hi,

I just want to know if it is possible to use one-step RT-PCR kits to amplify the DNA samples?

I have a one-step kit in the lab, but don't have any other normal PCR kits. just wondering if I could use it to amplify my DNA sample. as long as I don't have RNA in the sample then no cDNA will be made so it shouldn't be making any problem. I could even skip programming for cDNA making on the machine.....has anybody done this before?

[phage434]

This would probably work, but it seems a bit of a shame to waste the more expensive kit on a normal PCR.