PCR bands in NTC Control and Neg Control Lanes
Posted 02 March 2009 - 01:34 PM
Posted 02 March 2009 - 07:58 PM
Posted 03 March 2009 - 07:03 AM
Posted 03 March 2009 - 08:17 AM
It does sound like you have contamination issues. If you order all your supplies already sterile, you don't need to autoclave them. 35 cycles is kind of a lot, even if you have only a very small amount of contamination, 35 cycles will amplify it - maybe try to drop down to 30 cycles if you have a good strong band in your positives. Good luck.
Posted 03 March 2009 - 07:07 PM