4 replies to this topic

[Janice]

hi guys!

im currently doing a research proposal for my biotechnology course and im interested in making a thermotolerant yeast but then i dont know where to start

i hope you can help me....


thanks a lot

janice

[WHR]

Janice, on Mar 2 2009, 03:43 AM, said:

hi guys!

im currently doing a research proposal for my biotechnology course and im interested in making a thermotolerant yeast but then i dont know where to start

i hope you can help me....


thanks a lot

janice

Directed evolution?
Mutagen treatment, grow at 39 degree C, repeat the cycles.  You may want to elevate the temperature.

[Janice]

WHR, on Mar 2 2009, 04:57 AM, said:

Janice, on Mar 2 2009, 03:43 AM, said:

hi guys!

im currently doing a research proposal for my biotechnology course and im interested in making a thermotolerant yeast but then i dont know where to start

i hope you can help me....


thanks a lot

janice

Directed evolution?
Mutagen treatment, grow at 39 degree C, repeat the cycles.  You may want to elevate the temperature.

hi whr!

thanks for your reply. what mutagens can i use? can i just use plasmids for transformation?


thanks

[phage434]

Short wave UV exposure is probably easiest and safest (don't get a sunburn).  You will likely have to try a range of exposure times to find the sub-lethal exposures which cause mutations but not too much death.  If you have a Stratagene membrane cross linker (Stratalinker) that would be ideal, but any source of 254 nm UV would work.

[WHR]

As phage suggested.  Besides, there are many chemical mutagens.  
I think thermo tolerance is affected by multiple genes. Overexpression of certain heat shock proteins may have little effects. (?)