Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

mutation in secreted protein


  • Please log in to reply
5 replies to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 197 posts
2
Neutral

Posted 02 March 2009 - 01:44 AM

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA. My question is how do i chek for mutation in such a protein?

#2 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 197 posts
2
Neutral

Posted 02 March 2009 - 01:45 AM

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA. My question is how do i chek for mutation in such a protein?



#3 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 197 posts
2
Neutral

Posted 02 March 2009 - 01:47 AM

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA. My question is how do i chek for mutation in such a protein?



Do I have to check the mutation in the tissue sample corresponding to that serum sample showing dysregulated expression?

#4 WHR

WHR

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 02 March 2009 - 02:48 AM

Hi,
What kind of mutation do you expect to affect secretion of the protein? A mutation affecting expression level, or protein properties?

#5 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 197 posts
2
Neutral

Posted 02 March 2009 - 05:34 PM

expression level

#6 WHR

WHR

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 02 March 2009 - 11:19 PM

expression level


Expression level can be affected by multiple factors, for example, changes in promoter sequence, epigenetic modifications, upstream regulatory factors, ...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.