5 replies to this topic

[SF_HK]

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA.  My question is how do i chek for mutation in such a protein?

[SF_HK]

SF_HK, on Mar 2 2009, 05:44 PM, said:

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA.  My question is how do i chek for mutation in such a protein?

[SF_HK]

SF_HK, on Mar 2 2009, 05:44 PM, said:

Hi,

I'm measuring the expresson of a serum proetin (a secreted protein in serum) using ELISA.  My question is how do i chek for mutation in such a protein?

Do I have to check the mutation in the tissue sample corresponding to that serum sample showing dysregulated expression?

[WHR]

Hi,
What kind of mutation do you expect to affect secretion of the protein?  A mutation affecting expression level, or protein properties?

[SF_HK]

expression level

[WHR]

SF_HK, on Mar 2 2009, 05:34 PM, said:

expression level

Expression level can be affected by multiple factors, for example, changes in promoter sequence, epigenetic modifications, upstream regulatory factors, ...