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Storing HEK293 cells at -80C


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5 replies to this topic

#1 MaggieRoara

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Posted 01 March 2009 - 04:53 PM

I have some HEK 293 cells stored at -80C. My liquid nitro tank was full so i had to leave them at-80C. Can i still thaw and use them. How long do they remain viable at this temp?

#2 pcrman

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Posted 01 March 2009 - 06:03 PM

For short term storage, your cells are OK. Cells at -80C can be viable after months or even years.

#3 MaggieRoara

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Posted 01 March 2009 - 06:42 PM

For short term storage, your cells are OK. Cells at -80C can be viable after months or even years.



Thanks pcrman,

I have thawed them into media. I will change media at 4hours, so we'll see how it goes

#4 bob1

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Posted 02 March 2009 - 03:23 PM

They should be OK for a few weeks. Cells will survive up to about a year at -80, but the viability rapidly drops, so in effect you are selecting for cells that are resistant to freezing and/or poor conditions... meaning that your cells are no longer the same as the original cells you had.

The moral is: be careful what you do so as to not artificially change your cells and their responses.

#5 labrat612

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Posted 05 March 2009 - 09:52 AM

I generally keep my adherent HEK cells stored at -80C for up to 6 months. After that time, as previously said, their viability drops off as well as how they might behave.


~Labrat

#6 scolix

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Posted 07 March 2009 - 04:14 AM

I have kept some of my 293 cells for upto 1.5 years and they have been fine.




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