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normal PCR


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3 replies to this topic

#1 thinsu

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Posted 01 March 2009 - 06:10 AM

In normal PCR, two bands is found in only one sample clearly in the middle and at the end.Which is its result.?
Please explain me.Why it is caused?

#2 scolix

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Posted 01 March 2009 - 06:17 AM

In normal PCR, two bands is found in only one sample clearly in the middle and at the end.Which is its result.?
Please explain me.Why it is caused?


Its probably non-specific binding of primers which causes extra bands.

Does any of the bands correspond to the expected product size? If both bands are not right, then you need to optimize conditions for PCR like Mg cocentration, template, etc. .

#3 Felipillo

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Posted 01 March 2009 - 08:25 AM

Or may be could be supercoiled DNA, non-specific binding means low product, and many bands. You have also your template, it could be your other band, hope it helps.

Edited by Déjà Vu, 01 March 2009 - 08:27 AM.

Chance favors the prepared mind
Louis Pasteur.

#4 WHR

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Posted 01 March 2009 - 10:47 PM

In normal PCR, two bands is found in only one sample clearly in the middle and at the end.Which is its result.?
Please explain me.Why it is caused?


If the band at the end is less than 100 bp, it is likely primer dimers.




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