I managed to prepare 1 liter of 0.1M (pH 6.2) citrate buffer using citric acid and sodium citrate. Then i dissolved the required concentration of calcium chloride and the pH dropped to 4.6. I feel there is an error in my addition of CaCl2. what should i do to maintain the pH at 6.2?
Finally, can i do a POD assay without using PVPP as i see in other methods(currently i don't have it in stock )
Edited by kimeric, 28 February 2009 - 03:34 AM.