Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How does the protein fixation on SDS gel work?


  • Please log in to reply
3 replies to this topic

#1 Opal

Opal

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 30 posts
0
Neutral

Posted 27 February 2009 - 10:41 AM

Hi

I use 40% methanol, 10% acetic acid to fix proteins on the 2-D gels. Does anybody know what is the mechanism by which this fixation method work?

Thanks

Opal

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,784 posts
132
Excellent

Posted 02 March 2009 - 11:54 AM

the acetic acid fixes the protein similarly to the way tca does, except it is reversible. i can't give you the exact chemistry behind it, though. i may be able to find the answer from our resident organic chemist.
talent does what it can
genius does what it must
i do what i get paid to do

#3 genehunter

genehunter

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 106 posts
0
Neutral

Posted 02 March 2009 - 03:03 PM

It is probably just simple denaturation/precipitation in the presence of acid/MeOH. There is no covalent reaction taking place.

#4 Opal

Opal

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 30 posts
0
Neutral

Posted 06 March 2009 - 07:40 PM

Thanks,

This much of information is enough for me.

Opal




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.