3 replies to this topic

[Opal]

Hi

I use 40% methanol, 10% acetic acid to fix proteins on the 2-D gels. Does anybody know what is the mechanism by which this fixation method work?

Thanks

Opal

[mdfenko]

the acetic acid fixes the protein similarly to the way tca does, except it is reversible. i can't give you the exact chemistry behind it, though. i may be able to find the answer from our resident organic chemist.

[genehunter]

It is probably just simple denaturation/precipitation in the presence of acid/MeOH. There is no covalent reaction taking place.

[Opal]

Thanks,

This much of information is enough for me.

Opal