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lymphocytes


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#1 leroyrebello

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Posted 04 May 2002 - 02:54 AM

Can u tell me how to grow lymphocytes in culture .
As i need id for G-band staining...........Please..........its Urgent

#2 alloyd

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Posted 14 June 2002 - 09:40 AM

For lymphocyte culture try a polystyrene TC flask (Falcon makes them & sells thru fisher) with RPMI medium enriched with FBS or HSA, at a cell density not to exceed 500000 cells/ml.  In static culture these should last at least 24-30 hours.




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