Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

where to buy rotorgene real-time rt-pcr kits?


  • Please log in to reply
4 replies to this topic

#1 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 26 February 2009 - 09:32 AM

hi

I know that Qiagen supplies RT-PCR kits for rotorgene 6000, but their kit is for 400 reaction and is very expensive.

sigma, promega and Fermentas also have some kits but they don't say if the kits are specific for rotorgene or not.

where can i buy this kit?

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,501 posts
252
Excellent

Posted 27 February 2009 - 06:13 PM

As I understand it (no experience) the Rotorgene machine's capillaries require slightly higher magnesium concentration due to absorbtion onto the glass walls. You could use any standard PCR mix and increase the Mg++ salt concentration slightly to solve this problem.

#3 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 27 February 2009 - 07:01 PM

rotorgene glass wall?....I think you confused it with Lightcycler capillaries.....but thanks anyway...I didn't know this :D

#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,501 posts
252
Excellent

Posted 27 February 2009 - 07:23 PM

You're right, I was confusing it with the Lightcycler.

#5 NITIN SEMWAL

NITIN SEMWAL

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 09 March 2009 - 04:47 AM

Dear Curtis,

You can buy all these Rotor gene Kits at Genome Diagnostics Pvt. Ltd. website:

www.genomediagnostics.co.in

They are economical & very good quality

Nitin



hi

I know that Qiagen supplies RT-PCR kits for rotorgene 6000, but their kit is for 400 reaction and is very expensive.

sigma, promega and Fermentas also have some kits but they don't say if the kits are specific for rotorgene or not.

where can i buy this kit?






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.