4 replies to this topic

[Curtis]

hi

I know that Qiagen supplies RT-PCR kits for rotorgene 6000, but their kit is for 400 reaction and is very expensive.

sigma, promega and Fermentas also have some kits but they don't say if the kits are specific for rotorgene or not.

where can i buy this kit?

[phage434]

As I understand it (no experience) the Rotorgene machine's capillaries require slightly higher magnesium concentration due to absorbtion onto the glass walls.  You could use any standard PCR mix and increase the Mg++ salt concentration slightly to solve this problem.

[Curtis]

rotorgene glass wall?....I think you confused it with Lightcycler capillaries.....but thanks anyway...I didn't know this

[phage434]

You're right, I was confusing it with the Lightcycler.

[NITIN SEMWAL]

Dear Curtis,

You can buy all these Rotor gene Kits at Genome Diagnostics Pvt. Ltd. website:

www.genomediagnostics.co.in

They are economical & very good quality

Nitin

Curtis, on Feb 26 2009, 10:32 AM, said:

hi

I know that Qiagen supplies RT-PCR kits for rotorgene 6000, but their kit is for 400 reaction and is very expensive.

sigma, promega and Fermentas also have some kits but they don't say if the kits are specific for rotorgene or not.

where can i buy this kit?