Submit your paper to J Biol Methods today!

# Equation for calculating insert:vector ratio in ligation reaction

Started by sagar, Feb 26 2009 09:27 AM

5 replies to this topic

### #1

Posted 26 February 2009 - 09:27 AM

hi all

i am very much in need for the equations to carry out calculations before performing ligation, can any one plz help?

i am very much in need for the equations to carry out calculations before performing ligation, can any one plz help?

### #2

Posted 26 February 2009 - 08:03 PM

Hey

Equations? calculations? for ligation?

I am not sure if I understand what you mean to convey.

TC

Equations? calculations? for ligation?

I am not sure if I understand what you mean to convey.

TC

hi all

i am very much in need for the equations to carry out calculations before performing ligation, can any one plz help?

### #3

Posted 28 February 2009 - 08:54 AM

Do you need the ligation recipe or how to calculate the vector-insert ratio? Please be more specific.

### #5

Posted 05 March 2009 - 12:38 AM

insert mass in ng = 6 X (insert lenght in bp/vector lenght in bp) X vector mass in ng

You may change the 6 above depending on the actual ratio you want to get (sometimes a 3:1 works best than 6:1)

One thing to mention, you have to get pure vector and insert before applying this equation. Sometimes RNA contamination will lead to incorrect calculation.

Try 3:1 and 1:1 insert to vector ratio, these two ratio usually rock in ligation reaction.

### #6

Posted 08 March 2009 - 09:10 PM

Hi Sagar,

Go to the 'Biomath Calculator' website using the following link.

http://www.promega.c...ath/default.htm

Click on Ligations: Molar Ratio of Insert to Vector

Enter the parameters of your ligation and it will calculate the relative amounts for you.

Easy!

Go to the 'Biomath Calculator' website using the following link.

http://www.promega.c...ath/default.htm

Click on Ligations: Molar Ratio of Insert to Vector

Enter the parameters of your ligation and it will calculate the relative amounts for you.

Easy!