for the moment I need to detect expression of mRNA in our cells so definitely I would prefer to buy a one-step kit, but the question is that in case I finish working with mRNA and then decide to work with DNA sample, can I still use the same one-step RT-PCR kit? because the first cDNA snthesis step shouldn't be interfering with my DNA samples. am I right?...if not....then wouldn't it be better to buy a two-step kit?
I still haven't decided to buy from which company and I am aware that company issues are not for this subforum, but still I would appreciate if you guys could let me know where you buy your kits from?
Edited by Curtis, 26 February 2009 - 09:21 AM.